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High resolution imaging of IgG and IgM molecules by scanning tunneling microscopy in air condition

A scanning tunneling microscope is a powerful tool for obtaining micrographs from conductive and semiconductive materials. The imaging technique has recently been improved for microscopy of nanostructured biomaterials on highly ordered atomic surfaces. We describe, here, high resolution imaging of individual IgM and IgG using a scanning tunneling microscope (Nama-STM) in air condition. The biomolecules were immobilized on the surface of Highly Ordered Pyrolytic Graphite (HOPG). Obtained micrographs could reveal structural details of immunoglobulins G and M on the atomically flat surfaces. Obtained results confirmed that STM could be more useful than other microscopy techniques for the analysis of single biomolecules.

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